| Congresso Brasileiro de Microbiologia 2023 | Resumo: 409-1 | ||||
Resumo:The State of Bahia has the ninth largest bovine herd in the country, corresponding to 5.5% of all national production. Among the diseases that can affect the herd, bovine tuberculosis stands out for being a chronic zoonotic disease, which causes countless damages to public health and considerable economic losses to the world's beef producer and exporter market. One measure to assist in the surveillance and diagnosis of the disease is the detection of suspected tuberculosis lesions through the inspection system implemented in slaughterhouses, which forward post-mortem findings to specialized laboratories for confirmation of the Mycobacterium bovis agent through complementary tests. The objective of this study was to investigate the prevalence of bovine tuberculosis, through the application of the multiplex PCR technique and bacteriological isolation, in presumptive tuberculosis fragments found in slaughtered cattle slaughterhouses with official inspection in the state of Bahia. A total of 996,298 bovine carcasses were investigated with state and federal inspection services between the period of March 2020 and December 2021, among which 32 animals (0.0003%) had lesions suggestive of tuberculosis, where 42 samples were collected from 30 bovines. Of the 42 samples, 26.2% (11/42) showed growth in selective culture medium for M. bovis and 90.9% (10/11) of the smears showed alcohol-acid resistant bacilli (BAAR). BAAR isolates were submitted to multiplex PCR in which the presence of the agent M. bovis was confirmed in 100% (10/10) of the samples. This molecular technique, associated with post mortem and bacteriological examinations, revealed the presence of the disease in the state and enabled a more accurate diagnosis. The knowledge about these epidemiological indicators allows planning actions aimed at tracking, controlling and monitoring bovine tuberculosis, thus avoiding zoonotic outbreaks and economic losses with public health expenditure and disposal of infected animals.
Keyword: Epidemiological surveillance; Public health; Inspection; Mycobacterium bovis; Zoonosis
Development Agency: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – CAPES
Palavras-chave: Epidemiological surveillance, Inspection, Mycobacterium bovis, Public health, Zoonosis Agência de fomento:CAPES | |||||